Author: Lara Urban; Andre Holzer; J Jotautas Baronas; Michael Hall; Philipp Braeuninger-Weimer; Michael J Scherm; Daniel J Kunz; Surangi N Perera; Daniel E Martin-Herranz; Edward T Tipper; Susannah J Salter; Maximilian R Stammnitz
Title: Freshwater monitoring by nanopore sequencing Document date: 2020_2_7
ID: 77nsidzc_14
Snippet: In line with these physicochemical trends, we next determined the spatiotemporal enrichment of potentially functionally important bacterial taxa through nanopore sequencing. We retrieved 55 potentially pathogenic bacterial genera through careful integration of species known to affect human health 22, 23 , and also 13 wastewaterassociated 24 bacterial genera (Supplementary Table 3 ). Of these, 21 potentially pathogenic and eight wastewaterassociat.....
Document: In line with these physicochemical trends, we next determined the spatiotemporal enrichment of potentially functionally important bacterial taxa through nanopore sequencing. We retrieved 55 potentially pathogenic bacterial genera through careful integration of species known to affect human health 22, 23 , and also 13 wastewaterassociated 24 bacterial genera (Supplementary Table 3 ). Of these, 21 potentially pathogenic and eight wastewaterassociated genera were detected across all of the river samples (Figure 3c ; Methods). Many of these signals were stronger downstream of urban sections, within the mooring zone for recreational and residential barges (location 7, Figure 1a ) and in the vicinity of sewage outflow from a nearby wastewater treatment plant (location 8). The most prolific candidate pathogen genus observed was Arcobacter, which features multiple species implicated in acute gastrointestinal infections 25 .
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