Selected article for: "luciferase activity and room temperature"
Author: Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird
Title: A novel antiviral formulation inhibits a range of enveloped viruses.
  • Document date: 2020_3_30
    • ID: nly9vojr_10
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi. org/10.1101 org/10. /2020 Virus containing media was incubated with the indicated concentrations of ViroSAL, at pH5.5, for 2 minutes at room temperature unless stated otherwise, and an equal concentration of neutralizing buffer was added to restore pH to 7, and virus/ViroSAL solutions were added to target cells for 8 hours. Each preparation of ViroSAL was titra.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi. org/10.1101 org/10. /2020 Virus containing media was incubated with the indicated concentrations of ViroSAL, at pH5.5, for 2 minutes at room temperature unless stated otherwise, and an equal concentration of neutralizing buffer was added to restore pH to 7, and virus/ViroSAL solutions were added to target cells for 8 hours. Each preparation of ViroSAL was titrated in the relevant media used to culture each cell type (DMEM, Williams E and M199). To control for pH treatment, virus was treated with pH5.5 buffer that did not contain ViroSAL for 2 minutes and then pH restored to 7 as described for ViroSAL treatments and virus added to target cells. After 8 hours, unbound virus was removed and the media replaced with DMEM/3% FBS. At 72h post-infection the cells were lysed, luciferase substrate added (Promega, U.K.) and luciferase activity measured for 10 seconds in a luminometer (Lumat LB9507).

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