Selected article for: "chemical shift and imino proton"
Author: Neha Jain; Subodh Kumar Mishra; Uma Shankar; Arpita Tawani; Ankit Jaiswal; Tarun Kumar Sharma; Prashant Kodgire; Amit Kumar
Title: G-quadruplex stabilization in the ions and maltose transporters inhibit Salmonella enterica growth and virulence
  • Document date: 2018_6_27
    • ID: 57xx291v_23
    Snippet: NMR spectroscopy is considered as a most reliable technique for confirming the formation of Gquadruplex structure formation by the nucleic acid sequences. Therefore 1D 1 H NMR spectroscopy was performed to confirm the formation of G-quadruplex conformation by SE-PGQs. The presence of a chemical shift in the range of 10-12 ppm in 1D 1 H NMR spectra depicts the presence of hoogsteen base pairing in characteristic G-tetrads of G-quadruplex structure.....
    Document: NMR spectroscopy is considered as a most reliable technique for confirming the formation of Gquadruplex structure formation by the nucleic acid sequences. Therefore 1D 1 H NMR spectroscopy was performed to confirm the formation of G-quadruplex conformation by SE-PGQs. The presence of a chemical shift in the range of 10-12 ppm in 1D 1 H NMR spectra depicts the presence of hoogsteen base pairing in characteristic G-tetrads of G-quadruplex structure whereas, canonical G-C Watson Crick base pairing can be characterized by a chemical shift in the range of 12-14 ppm. All the three SE-PGQs showed an imino proton resonance between 10-12 ppm and clearly affirms the formation of G-quadruplex structure (Fig 4 & S1 Fig) .

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