Author: Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird
Title: A novel antiviral formulation inhibits a range of enveloped viruses. Document date: 2020_3_30
ID: nly9vojr_14
Snippet: IC323 carrying the open reading frame of the enhanced green fluorescent protein (EGFP) was generated as described previously (Hashimoto et al., 2002) . After initial recovery, MeV was produced in Vero-SLAM cells using an initial multiplicity of infection of 0.1. When infection was fully developed, flasks were vigorously shaken and supernatants collected and clarified at 3500rpm for 30min at 4â°C. Supernatant was collected, aliquoted and stored a.....
Document: IC323 carrying the open reading frame of the enhanced green fluorescent protein (EGFP) was generated as described previously (Hashimoto et al., 2002) . After initial recovery, MeV was produced in Vero-SLAM cells using an initial multiplicity of infection of 0.1. When infection was fully developed, flasks were vigorously shaken and supernatants collected and clarified at 3500rpm for 30min at 4â°C. Supernatant was collected, aliquoted and stored at -80â°C. the author/funder. All rights reserved. No reuse allowed without permission.
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