Author: Lara Urban; Andre Holzer; J Jotautas Baronas; Michael Hall; Philipp Braeuninger-Weimer; Michael J Scherm; Daniel J Kunz; Surangi N Perera; Daniel E Martin-Herranz; Edward T Tipper; Susannah J Salter; Maximilian R Stammnitz
Title: Freshwater monitoring by nanopore sequencing Document date: 2020_2_7
ID: 77nsidzc_22
Snippet: Most routine freshwater surveillance frameworks focus on semi-quantitative diagnostics of only a limited number of target taxa, such as pathogenic Salmonella, Legionella and faecal coliforms 33, 34 . Our proof-of-principle analysis highlights that the combination of full-length 16S rRNA gene amplification and nanopore sequencing can complement hydrochemical controls in pinpointing relatively contaminated freshwater sites, some of which had been p.....
Document: Most routine freshwater surveillance frameworks focus on semi-quantitative diagnostics of only a limited number of target taxa, such as pathogenic Salmonella, Legionella and faecal coliforms 33, 34 . Our proof-of-principle analysis highlights that the combination of full-length 16S rRNA gene amplification and nanopore sequencing can complement hydrochemical controls in pinpointing relatively contaminated freshwater sites, some of which had been previously highlighted for their pathogen diversity and abundance of antimicrobial resistance genes 35, 36 .
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