Author: Lara Urban; Andre Holzer; J Jotautas Baronas; Michael Hall; Philipp Braeuninger-Weimer; Michael J Scherm; Daniel J Kunz; Surangi N Perera; Daniel E Martin-Herranz; Edward T Tipper; Susannah J Salter; Maximilian R Stammnitz
Title: Freshwater monitoring by nanopore sequencing Document date: 2020_2_7
ID: 77nsidzc_40
Snippet: DNA extracts from each sampling batch and DI water control were separately amplified with V1-V9 full-length Figure 1b-c) , which was previously assessed using nanopore shotgun metagenomics 42 . We used common primer binding sequences 27f and 1492r, both coupled to unique 24 bp barcodes and a nanopore motor protein tether sequence (Supplementary Table 7 ). Fulllength 16S rDNA PCRs were performed with the following conditions: Table S2b ). We used .....
Document: DNA extracts from each sampling batch and DI water control were separately amplified with V1-V9 full-length Figure 1b-c) , which was previously assessed using nanopore shotgun metagenomics 42 . We used common primer binding sequences 27f and 1492r, both coupled to unique 24 bp barcodes and a nanopore motor protein tether sequence (Supplementary Table 7 ). Fulllength 16S rDNA PCRs were performed with the following conditions: Table S2b ). We used KAPA Pure Beads (KAPA Biosystems, Wilmington, MA, USA) to concentrate full-length 16S rDNA products in 21 µL DI water. Multiplexed nanopore ligation sequencing libraries were then made by following the SQK-LSK109 protocol (Oxford Nanopore Technologies, Oxford, UK).
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