Author: James T. Van Leuven; Martina M. Ederer; Katelyn Burleigh; LuAnn Scott; Randall A. Hughes; Vlad Codrea; Andrew D. Ellington; Holly Wichman; Craig Miller
Title: FX174 Attenuation by Whole Genome Codon Deoptimization Document date: 2020_2_11
ID: mpb4fy16_54
Snippet: Fitness assays and fitness calculations were performed as previously described (27) . The assay is a determination of growth rate at low MOI in 10 mL LB and is carried out at 37°C. Host cells were prepared by growing to~10 8 cells/mL and aliquoted into 8.5 mL of warm LB just prior to adding phage. Phage fitness is expressed as the log2 fold increase in the total number of phage per hour. All measurements were done in triplicate. At 45min, virus .....
Document: Fitness assays and fitness calculations were performed as previously described (27) . The assay is a determination of growth rate at low MOI in 10 mL LB and is carried out at 37°C. Host cells were prepared by growing to~10 8 cells/mL and aliquoted into 8.5 mL of warm LB just prior to adding phage. Phage fitness is expressed as the log2 fold increase in the total number of phage per hour. All measurements were done in triplicate. At 45min, virus titers were done on LB-agar plates with 0.3% top agar.
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