G-quadruplex stabilization in the ions and maltose transporters inhibit Salmonella enterica growth and virulence

Neha Jain; Subodh Kumar Mishra; Uma Shankar; Arpita Tawani; Ankit Jaiswal; Tarun Kumar Sharma; Prashant Kodgire; Amit Kumar

CD spectra analysis revealed the predominant parallel G-quadruplex topology exhibited by SE-PGQ-1 and SE-PGQ-3 in the presence of the K + ion, whereas SE-PGQ-2 showed hybrid G-quadruplex topology in the presence of K + (Fig 5a) . As expected, CD spectral scanning performed in the increasing concentration of K + ion showed the maximum molar ellipticity in highest K + ion concentration ( The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/357046 doi: bioRxiv preprint Adenine and CD spectra analysis was performed in 50 mM K + ion (S4 Table) . Mutants (mut-PGQ-1, mut-PGQ-2, and mut-PGQ-3) failed to show the characteristic CD signal of G-quadruplex i.e. a positive band at 260/290 nm and a negative band at 240 nm suggesting the mutation in G tract disrupted the Gquadruplex formation (Fig 5a) .