Author: Peroni, L.; Toscaro, J.; Canateli, C.; Lima, G.; Pagliarone, a.; Cardoso, E.; Viana, R.; Bassani, J.; Lin-Wang, H.; Abboud, C. S.; Gun, C.; Franchini, K.; Bajgelman, M. C.
Title: Development of low-cost serological immunoassay to detect antiviral antibodies to Sars-Cov-2 Spike protein Cord-id: 8u7ii4kg Document date: 2021_6_9
ID: 8u7ii4kg
Snippet: Seroconversion to Sars-cov-2 has been widely studied to evaluate infection spreading for epidemiological purpose, or even in studies of protective immunity in convalescent or vaccinated individuals. The viral particle has an envelope harboring the SPIKE glycoprotein, which can be used as an antigen for assay development, to detect antiviral antibodies to Sars-CoV-2. Since several vaccines encode a SPIKE subunit, the full length spike-based immunoassay should be a universal tool to evaluate seroc
Document: Seroconversion to Sars-cov-2 has been widely studied to evaluate infection spreading for epidemiological purpose, or even in studies of protective immunity in convalescent or vaccinated individuals. The viral particle has an envelope harboring the SPIKE glycoprotein, which can be used as an antigen for assay development, to detect antiviral antibodies to Sars-CoV-2. Since several vaccines encode a SPIKE subunit, the full length spike-based immunoassay should be a universal tool to evaluate seroconversion. In this manuscript, we propose a low-cost assay that can be used to detect antiviral IgG to Sars-Cov-2 in human serum.
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