Generating genomic platforms to study Candida albicans pathogenesis

Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert

CandidaOrfDB was created to integrate the data of the C. albicans ORFeome project and is available at http://candidaorfeome.eu. CandidaOrfDB enables the scientific community to search for availability and quality of the clones. All data pertinent to the cloning process are stored in the database. This information includes the name and sequence of the primers used to amplify the ORFs from C. albicans SC5314 genomic DNA, the coordinates of the primers in their 96-well storage plates, and any sequencing information available on the clones. SNPs and DIPs, their location in the ORF and their influence on the corresponding amino acid sequences are shown (Fig. 2) . Nucleotide sequence of the cloned ORF and the corresponding amino acid sequence are displayed with highlights of SNPs or DIPs relative to the closest haplotype sequence (Fig. 2) . The reference sequence data have been extracted from CGD (46) . The database can be queried through the ORF number (orf19.xxxx or 19.xxxx; (5)), the Assembly 22 name (Ci_XXXXXW or Ci_XXXXXC; (5)) or the gene name.