Generating genomic platforms to study Candida albicans pathogenesis

Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/261628 doi: bioRxiv preprint possible mating of diploids, and generation of tetraploids, the absence of meiosis and the reversion to the diploid state by chromosome loss (reviewed in (51)). An essential prerequisite to mating in C. albicans is the phenotypic switch from white to opaque, a stable but reversible switch regulated by environmental stimuli such as low temperatures, carbon dioxide and nutrients. The epigenetic switch is tightly linked to mating since the a1/α2 complex, encoded at the mating type like (MTL) loci, acts as a repressor of opaque switching (52) . In that context, the two-hybrid strain SC2H3 originating from the diploid a/α SN152 strain was deleted for the MTLa or MTLα locus to construct SC2H3α and SC2H3a strains.