Selected article for: "bacterial pathogen and dna sequencing"
Author: Lara Urban; Andre Holzer; J Jotautas Baronas; Michael Hall; Philipp Braeuninger-Weimer; Michael J Scherm; Daniel J Kunz; Surangi N Perera; Daniel E Martin-Herranz; Edward T Tipper; Susannah J Salter; Maximilian R Stammnitz
Title: Freshwater monitoring by nanopore sequencing
  • Document date: 2020_2_7
    • ID: 77nsidzc_2
    Snippet: In recent years, these challenges have been revisited with the prospect of 'portable' DNA analysis. The main driver of this is the smartphone-sized MinION device from Oxford Nanopore Technologies (ONT), which enables real-time DNA sequencing using nanopores 6 . Nanopore read lengths can be comparably long (currently up to ~2*10 6 bases 7 ), which is enabled by continuous electrical sensing of sequential nucleotides along single DNA strands. In co.....
    Document: In recent years, these challenges have been revisited with the prospect of 'portable' DNA analysis. The main driver of this is the smartphone-sized MinION device from Oxford Nanopore Technologies (ONT), which enables real-time DNA sequencing using nanopores 6 . Nanopore read lengths can be comparably long (currently up to ~2*10 6 bases 7 ), which is enabled by continuous electrical sensing of sequential nucleotides along single DNA strands. In connection with a laptop or cloud access for the translation of raw voltage signal into nucleotides, nanopore sequencing can be used to rapidly monitor long DNA sequences in remote locations. Although there are still common concerns about the technology's base-level accuracy, mobile MinION setups have already proven powerful for real-time tracing and open data sharing during bacterial and viral pathogen outbreaks [8] [9] [10] [11] [12] [13] .

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