Author: Wang, Deguo; Wang, Yongzhen; Zhang, Meng; Zhang, Yongqing; Sun, Juntao; Song, Chumei; Xiao, Fugang; Ping, Yuan; Pan, Chen; Hu, Yushan; Wang, Chaoqun; Liu, Yanhong
Title: Isothermal Amplification of Nucleic Acids with Ladder-shape Melting Curve Cord-id: n19zw8cu Document date: 2020_12_18
ID: n19zw8cu
Snippet: A novel method termed isothermal amplification of nucleic acids with ladder-shape melting curve (LMCP) was developed in the study. In this method, one pair of primers or two pairs of nested primers and a thermostable DNA polymerase (large fragment) were employed to amplify the internal transcribed spacer (ITS) of Oryza sativa with ladder-shape melting curve. Our results demonstrated that the LMCP assay with nested primers was 50-fold more sensitive and one-hour faster than the LAMP assay with th
Document: A novel method termed isothermal amplification of nucleic acids with ladder-shape melting curve (LMCP) was developed in the study. In this method, one pair of primers or two pairs of nested primers and a thermostable DNA polymerase (large fragment) were employed to amplify the internal transcribed spacer (ITS) of Oryza sativa with ladder-shape melting curve. Our results demonstrated that the LMCP assay with nested primers was 50-fold more sensitive and one-hour faster than the LAMP assay with the same level of specificity. The LMCP method has the potential to be used for the prevention and control of the emerging epidemics.
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