Author: Lara Urban; Andre Holzer; J Jotautas Baronas; Michael Hall; Philipp Braeuninger-Weimer; Michael J Scherm; Daniel J Kunz; Surangi N Perera; Daniel E Martin-Herranz; Edward T Tipper; Susannah J Salter; Maximilian R Stammnitz
Title: Freshwater monitoring by nanopore sequencing Document date: 2020_2_7
ID: 77nsidzc_45
Snippet: In collaboration with Public Health England, raw water DNA isolates of the River Cam from each location and time point were subjected to the UK reference service for leptospiral testing (Supplementary Table 5 ). This test is based on quantitative real-time PCR (qPCR) of 16S rDNA and LipL32, implemented as a TaqMan assay for the detection and differentiation of pathogenic and non-pathogenic Leptospira spp. from human serum. Briefly, the assay cons.....
Document: In collaboration with Public Health England, raw water DNA isolates of the River Cam from each location and time point were subjected to the UK reference service for leptospiral testing (Supplementary Table 5 ). This test is based on quantitative real-time PCR (qPCR) of 16S rDNA and LipL32, implemented as a TaqMan assay for the detection and differentiation of pathogenic and non-pathogenic Leptospira spp. from human serum. Briefly, the assay consists of a two-component PCR; the first component is a duplex assay that targets the gene encoding the outer membrane lipoprotein LipL32, which is reported to be strongly associated with the pathogenic phenotype.
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