Author: Haile, Simon; Nikiforuk, Aidan M.; Pandoh, Pawan K.; Twa, David D.W.; Smailus, Duane E.; Nguyen, Jason; Pleasance, Stephen; Wong, Angus; Zhao, Yongjun; Eisler, Diane; Moksa, Michelle; Cao, Qi; Wong, Marcus; Su, Edmund; Krzywinski, Martin; Nelson, Jessica; Mungall, Andrew J.; Tsang, Frankie; Prentice, Leah M.; Jassem, Agatha; Manges, Amee R.; Jones, Steven J.M.; Coope, Robin J.; Prystajecky, Natalie; Marra, Marco A.; Krajden, Mel; Hirst, Martin
Title: Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents Cord-id: hy4pqbe4 Document date: 2021_10_20
ID: hy4pqbe4
Snippet: The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
Document: The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
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