Author: Katherine E. Arden; Ristan M. Greer; Claire Y.T. Wang; Ian M. Mackay
Title: Genotypic diversity, circulation patterns, and co-detections among rhinoviruses in Queensland, 2001 Document date: 2018_5_30
ID: d0w40mkn_6
Snippet: Extracts had been previously tested by PQ using direct or culture-amplified direct fluorescent assay to detect respiratory syncytial virus (RSV), adenoviruses (AdV), parainfluenza viruses (PIVs) and influenza viruses A & B (IFAV, IFBV). 9 PCR was not in routine use at PQ in 2001. This study applied additional previously described realtime RT-PCR (RT-rtPCR) assays to detect human metapneumovirus (MPV) 8, 10 and human coronaviruses (HCoV) 229E, HKU.....
Document: Extracts had been previously tested by PQ using direct or culture-amplified direct fluorescent assay to detect respiratory syncytial virus (RSV), adenoviruses (AdV), parainfluenza viruses (PIVs) and influenza viruses A & B (IFAV, IFBV). 9 PCR was not in routine use at PQ in 2001. This study applied additional previously described realtime RT-PCR (RT-rtPCR) assays to detect human metapneumovirus (MPV) 8, 10 and human coronaviruses (HCoV) 229E, HKU1, NL63 and OC43. 11 RVs were detected using a modified version of a previously described RT-rtPCR. 12, 13 The RV RT-rtPCR can also detect some human enterovirus (EV) types.
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