Selected article for: "HCoV infection and MERS cov"
Author: Laing, Eric; Sterling, Spencer; Richard, Stephanie; Epsi, Nusrat; Phogat, Shreshta; Samuels, Emily; Yan, Lianying; Moreno, Nicole; Coles, Christian; Drew, Matthew; Mehalko, Jennifer; English, Caroline; Merritt, Scott; Mende, Katrin; Chung, Kevin; Clifton, G.; Munster, Vincent; de Wit, Emmie; Tribble, David; Agan, Brian; Esposito, Dominic; Lanteri, Charlotte; Mitre, Edward; Burgess, Timothy; Broder, Chritopher
Title: A betacoronavirus multiplex microsphere immunoassay detects early SARS-CoV-2 seroconversion and antibody cross reactions
  • Cord-id: s97s7xq5
  • Document date: 2020_11_24
    • ID: s97s7xq5
    Snippet: Sensitive and specific SARS-CoV-2 antibody assays remain critical for community and hospital-based SARS-CoV-2 surveillance. Here, we developed and applied a multiplex microsphere-based immunoassay (MMIA) for COVD-19 antibody studies that incorporates spike protein trimers of SARS-CoV-2, SARS-CoV-1, MERS-CoV, and the seasonal human betacoronaviruses, HCoV-HKU1 and HCoV-OC43, that enables measurement of off-target pre-existing cross-reactive antibodies. The MMIA performances characteristics are: 9
    Document: Sensitive and specific SARS-CoV-2 antibody assays remain critical for community and hospital-based SARS-CoV-2 surveillance. Here, we developed and applied a multiplex microsphere-based immunoassay (MMIA) for COVD-19 antibody studies that incorporates spike protein trimers of SARS-CoV-2, SARS-CoV-1, MERS-CoV, and the seasonal human betacoronaviruses, HCoV-HKU1 and HCoV-OC43, that enables measurement of off-target pre-existing cross-reactive antibodies. The MMIA performances characteristics are: 98% sensitive and 100% specific for human subject samples collected as early as 10 days from symptom onset. The MMIA permitted the simultaneous identification of SARS-CoV-2 seroconversion and the induction of SARS-CoV-2 IgG antibody cross reactions to SARS-CoV-1 and MERS-CoV. Further, synchronous increases of HCoV-OC43 IgG antibody levels was detected with SARS-CoV-2 seroconversion in a subset of subjects for whom early infection sera were available prior to their SARS-CoV-2 seroconversion, suggestive of an HCoV-OC43 memory response triggered by SARS-CoV-2 infection.

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