Selected article for: "amplified sequence and PCR product"
Author: Mélanie Legrand; Sophie Bachellier-Bassi; Keunsook K. Lee; Yogesh Chaudhari; Hélène Tournu; Laurence Arbogast; Hélène Boyer; Murielle Chauvel; Vitor Cabral; Corinne Maufrais; Audrey Nesseir; Irena Maslanka; Emmanuelle Permal; Tristan Rossignol; Louise A. Walker; Ute Zeidler; Sadri Znaidi; Floris Schoeters; Charlotte Majgier; Renaud A. Julien; Laurence Ma; Magali Tichit; Christiane Bouchier; Patrick Van Dijck; Carol A. Munro; Christophe d’Enfert
Title: Generating genomic platforms to study Candida albicans pathogenesis
  • Document date: 2018_2_8
    • ID: 1vx62ofn_25
    Snippet: Accession numbers are given in Table 2 . All oligonucleotides used for PCR and/or sequencing are listed in Supplemental Table 2. SP cloning. First, a spacer sequence (SP) was amplified from the E. coli kanR gene borne on pCR-topo-blunt plasmid (Invitrogen) with primers SP3 and SP4. The PCR product was cloned in the SacII site of CIp-P TET -GTW, yielding CIp-P TET -GTW-SP, also referred to as pCA-Dest1100. This 390 bp-long sequence is flanked by t.....
    Document: Accession numbers are given in Table 2 . All oligonucleotides used for PCR and/or sequencing are listed in Supplemental Table 2. SP cloning. First, a spacer sequence (SP) was amplified from the E. coli kanR gene borne on pCR-topo-blunt plasmid (Invitrogen) with primers SP3 and SP4. The PCR product was cloned in the SacII site of CIp-P TET -GTW, yielding CIp-P TET -GTW-SP, also referred to as pCA-Dest1100. This 390 bp-long sequence is flanked by the SP1 and SP2 Illumina pairedend sequencing primers 1 and 2 and can be used to insert molecular barcodes if expression plasmids are to be used in signature-tagged mutagenesis approaches.

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