Author: Jang, Hongje; Kim, Youngâ€Kwan; Kwon, Hyunâ€Mi; Yeo, Woonâ€Seok; Kim, Dongâ€Eun; Min, Dalâ€Hee
Title: A Grapheneâ€Based Platform for the Assay of Duplexâ€DNA Unwinding by Helicase Cord-id: ozlednrj Document date: 2010_7_7
ID: ozlednrj
Snippet: Time to unwind: Graphene oxide (GO) enables the quantitative measurement of helicaseâ€dependent doubleâ€stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescentâ€dyeâ€labeled singleâ€stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.[Image: see text]
Document: Time to unwind: Graphene oxide (GO) enables the quantitative measurement of helicaseâ€dependent doubleâ€stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescentâ€dyeâ€labeled singleâ€stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.[Image: see text]
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