Electrical probing of COVID-19 spike protein receptor binding domain via a graphene field-effect transistor

Zhang, Xiaoyan; Qi, Qige; Jing, Qiushi; Ao, Shen; Zhang, Zhihong; Ding, Mingchao; Wu, Muhong; Liu, Kaihui; Wang, Weipeng; Ling, Yunhan; Zhang, Zhengjun; Fu, Wangyang

Here, in an effort towards facile and fast screening/diagnosis of novel coronavirus disease 2019 (COVID-19), we combined the unprecedently sensitive graphene field-effect transistor (Gr-FET) with highly selective antibody-antigen interaction to develop a coronavirus immunosensor. The Gr-FET immunosensors can rapidly identify (about 2 mins) and accurately capture the COVID-19 spike protein S1 (which contains a receptor binding domain, RBD) at a limit of detection down to 0.2 pM, in a real-time and label-free manner. Further results ensure that the Gr-FET immunosensors can be promisingly applied to screen for high-affinity antibodies (with binding constant up to 2*10^11 M^-1 against the RBD) at concentrations down to 0.1 pM. Thus, our developed electrical Gr-FET immunosensors provide an appealing alternative to address the early screening/diagnosis as well as the analysis and rational design of neutralizing-antibody locking methods of this ongoing public health crisis.