Author: Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird
Title: A novel antiviral formulation inhibits a range of enveloped viruses. Document date: 2020_3_30
ID: nly9vojr_9
Snippet: Pseudoviruses were generated by transfecting 293T cells with plasmids encoding a HIV-1 provirus expressing luciferase (pNL4-3-Luc-R-E-) and vesicular stomatitis virus (VSV-G), Zika, Ebola, Lassa or SARS coronavirus-1 (SARS-CoV-1) envelope or a no envelope control (Env-), as previously described (Fletcher et al., 2015 (Broer et al., 2006 . Zika virus pseudoviruses were generated by amplifying preM-M-Env region (positions 753-3549) from the PE243 B.....
Document: Pseudoviruses were generated by transfecting 293T cells with plasmids encoding a HIV-1 provirus expressing luciferase (pNL4-3-Luc-R-E-) and vesicular stomatitis virus (VSV-G), Zika, Ebola, Lassa or SARS coronavirus-1 (SARS-CoV-1) envelope or a no envelope control (Env-), as previously described (Fletcher et al., 2015 (Broer et al., 2006 . Zika virus pseudoviruses were generated by amplifying preM-M-Env region (positions 753-3549) from the PE243 Brazil Zika strain (Accession No. KX197192), with flanking restriction sites 5'NheI-insert-XbaI-3' then inserting into pcDNA3.1(+) plasmid. the author/funder. All rights reserved. No reuse allowed without permission.
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