Gut microbiota structure differs between honey bees in winter and summer

Lucie Kešnerová; Olivier Emery; Michaël Troilo; Joanito Liberti; Berra Erkosar; Philipp Engel

of 750 μl corresponding to the sample volume of one 158 bee gut was transferred to a new 2 ml bead beating tube with glass beads and homogenized 159 again. All further steps of the DNA extraction were performed as previously described [6] . plasmid copies. For these targets, the Cq value corresponding to 10 2 copies was set as the 173 limit of detection (LOD) of the primer set, because dilutions containing 10 1 copies resulted 174 in Cq values which could not be discriminated from the water control, or the signal was 175 undetected. For all other targets (Gilliamella, Bifidobacterium, and actin), the slope and 176 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/703512 doi: bioRxiv preprint intercept of the standard curve was calculated based on the Cq values obtained from all 177 seven dilutions, and the Cq value corresponding to 10 1 copies was used as the LOD.