Selected article for: "cDNA dna and end repair"
Author: Jing Lu; Louis du Plessis; Zhe Liu; Verity Hill; Min Kang; Huifang Lin; Jiufeng Sun; Sarah Francois; Moritz U G Kraemer; Nuno R Faria; John T McCrone; Jinju Peng; Qianling Xiong; Runyu Yuan; Lilian Zeng; Pingping Zhou; Chuming Liang; Lina Yi; Jun Liu; Jianpeng Xiao; Jianxiong Hu; Tao Liu; Wenjun Ma; Wei Li; Juan Su; Huanying Zheng; Bo Peng; Shisong Fang; Wenzhe Su; Kuibiao Li; Ruilin Sun; Ru Bai; Xi Tang; Minfeng Liang; Josh Quick; Tie Song; Andrew Rambaut; Nick Loman; Jayna Raghwani; Oliver Pybus; Changwen Ke
Title: Genomic epidemiology of SARS-CoV-2 in Guangdong Province, China
  • Document date: 2020_4_4
    • ID: ju9japd8_44
    Snippet: The copyright holder for this preprint . https: //doi.org/10.1101 //doi.org/10. /2020 samples were measured and checked with the Agilent Bioanalyzer 2100 and Qubit. Libraries were prepared using the SMARTer Stranded Total Cat. No. 634412) according to the manufacturer's protocol starting with 10 ng total RNA. Briefly, purified RNA was firstly fragmented and converted to cDNA using reverse transcriptase. The ribosome cDNA was depleted by using Zap.....
    Document: The copyright holder for this preprint . https: //doi.org/10.1101 //doi.org/10. /2020 samples were measured and checked with the Agilent Bioanalyzer 2100 and Qubit. Libraries were prepared using the SMARTer Stranded Total Cat. No. 634412) according to the manufacturer's protocol starting with 10 ng total RNA. Briefly, purified RNA was firstly fragmented and converted to cDNA using reverse transcriptase. The ribosome cDNA was depleted by using ZapRv2 (mammalian-specific). The remaining cDNA was converted to double stranded DNA and subjected to end-repair, A-tailing, and adapter ligation. The constructed libraries were amplified using 9-16 PCR cycles. Sequencing of metatranscriptome libraries was conducted using Illumina NextSeq 550 SE 75 or BGI MGISEQ-2000 PE100 platforms.

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