Selected article for: "Cq value and genome sequence"

Author: Tsuyoshi Sekizuka; Kentaro Itokawa; Tsutomu Kageyama; Shinji Saito; Ikuyo Takayama; Hideki Asanuma; Nao Naganori; Rina Tanaka; Masanori Hashino; Takuri Takahashi; Hajime Kamiya; Takuya Yamagishi; Kensaku Kakimoto; Motoi Suzuki; Hideki Hasegawa; Takaji Wakita; Makoto Kuroda
Title: Haplotype networks of SARS-CoV-2 infections in the Diamond Princess cruise ship outbreak
  • Document date: 2020_3_27
  • ID: 7vuejy26_16
    Snippet: A) Box plots of the Cq value from RT-qPCR assays that either successfully resulted in whole 5 genome sequencing or incomplete sequencing. A Cq of at least 32 (~300 viral copy) is favorable for obtaining the whole genome sequence by PrimalSeq. B) The genome position of genetic variations found among DP isolates compared with the isolate Wuhan-Hu-1 (MN908947). C) The detected nucleotide transition and transversion in all GISAID available SARS-CoV-2.....
    Document: A) Box plots of the Cq value from RT-qPCR assays that either successfully resulted in whole 5 genome sequencing or incomplete sequencing. A Cq of at least 32 (~300 viral copy) is favorable for obtaining the whole genome sequence by PrimalSeq. B) The genome position of genetic variations found among DP isolates compared with the isolate Wuhan-Hu-1 (MN908947). C) The detected nucleotide transition and transversion in all GISAID available SARS-CoV-2 genomes (n= 248, see Supplementary Table S3 . Updated on March 10, 2020) and DP isolates 10 (n=73) compared with the isolate Wuhan-Hu-1 (MN908947). D) The detected ratio of synonymous/non-synonymous mutations on coding sequences or intergenic regions in all GISAID available SARS-CoV-2 genomes (n= 248, Updated on March 10, 2020) and DP isolates (n=73) compared with the isolate Wuhan-Hu-1 (MN908947).

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