Author: Liu, Weiping; Guo, Xiaolong; Deng, Jianping; Song, Yaohui; Yin, Minggang; Zhang, Chengli; Long, Xia; Cao, Chengjian
Title: Unmatched Virus Preservation Solution may Impede One-Step Reagent for Detection of 2019-nCoV RNA. Cord-id: v2twwnpm Document date: 2021_2_1
ID: v2twwnpm
Snippet: BACKGROUND AND METHODS 2019 Corona Virus Disease (COVID-19) caused by SARS-CoV-2 is still pandemic now. RT-qPCR detection was the most common method for the diagnosis of SARS-CoV-2 infection, facilitated by amounts of nucleic acid testing kits. However, the accuracy of nucleic acid detection is affected by various factors such as specimen collection, specimen preparation, reagents deficiency, and personnel quality. RESULTS In this study, we found that unmatched virus preservation solution will i
Document: BACKGROUND AND METHODS 2019 Corona Virus Disease (COVID-19) caused by SARS-CoV-2 is still pandemic now. RT-qPCR detection was the most common method for the diagnosis of SARS-CoV-2 infection, facilitated by amounts of nucleic acid testing kits. However, the accuracy of nucleic acid detection is affected by various factors such as specimen collection, specimen preparation, reagents deficiency, and personnel quality. RESULTS In this study, we found that unmatched virus preservation solution will inhibit N gene and OFR-1ab gene (two independent genes of SARS-CoV-2) amplification in one-step detection reagent. CONCLUSIONS Despite just being a particular phenomenon we found in our work to fight 2019-nCoV, we concluded that unmatched virus preservation solution may have an inhibitory effect on SARS-CoV-2 nucleic acid detection which may lead to incorrect clinical diagnosis.
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