Selected article for: "MERS CoV and negative control"
Author: Ting Gao; Mingdong Hu; Xiaopeng Zhang; Hongzhen Li; Lin Zhu; Hainan Liu; Qincai Dong; Zhang Zhang; Zhongyi Wang; Yong Hu; Yangbo Fu; Yanwen Jin; Kaitong Li; Songtao Zhao; Yongjiu Xiao; Shuping Luo; Lufeng Li; Lingfang Zhao; Junli Liu; Huailong Zhao; Yue Liu; Weihong Yang; Jing Peng; Xiaoyu Chen; Ping Li; Yaoning Liu; Yonghong Xie; Jibo Song; Lu Zhang; Qingjun Ma; Xiuwu Bian; Wei Chen; Xuan Liu; Qing Mao; Cheng Cao
Title: Highly pathogenic coronavirus N protein aggravates lung injury by MASP-2-mediated complement over-activation
  • Document date: 2020_3_30
    • ID: dxs8ggyh_33
    Snippet: As described previously (40) , pET22b-SARS/MERS-CoV N was transformed into the expression strain BL21 (DE3). After induction with 1 mM IPTG for 8 h, the bacteria were harvested by centrifugation and re-suspended in buffer A (25 mM Na 2 HPO 4 /NaH 2 PO 4 (pH 8.0), 35 1 mM EDTA, and 1 mM DTT) before sonication. Soluble N protein in the lysate was purified with ion-exchange chromatography with SP-Sepharose Fast Flow (25 mM Na 2 HPO 4 /NaH 2 PO 4 (pH.....
    Document: As described previously (40) , pET22b-SARS/MERS-CoV N was transformed into the expression strain BL21 (DE3). After induction with 1 mM IPTG for 8 h, the bacteria were harvested by centrifugation and re-suspended in buffer A (25 mM Na 2 HPO 4 /NaH 2 PO 4 (pH 8.0), 35 1 mM EDTA, and 1 mM DTT) before sonication. Soluble N protein in the lysate was purified with ion-exchange chromatography with SP-Sepharose Fast Flow (25 mM Na 2 HPO 4 /NaH 2 PO 4 (pH 8.0), 1 mM EDTA, 1 mM DTT, and 0.35-0.5 M NaCl), followed by Superdex 200 gel filtration (GE Healthcare) and elution with buffer A. E. coli transformed with the vector pET22b was lysed as described above, and the eluate was used as a negative control for the purified N Purification and renaturation of MASP-2

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