Author: Mahesh K R Kalikiri; Mohammad Rubayet Hasan; Faheem Mirza; Thabisile Xaba; Patrick Tang; Stephan Lorenz
Title: High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads Document date: 2020_4_11
ID: bx2xspbe_21
Snippet: The copyright holder for this preprint . https://doi.org/10.1101/2020.04.08.20055731 doi: medRxiv preprint directly added to the RT-qPCR reaction. Until more experiments are done to fully understand the intricacies of this effect, we opted for a compromise between increased capture efficiency and consistency and a weak suppressive effect on the reverse transcription efficiency. When applying these conditions to 204 clinical samples, we only obser.....
Document: The copyright holder for this preprint . https://doi.org/10.1101/2020.04.08.20055731 doi: medRxiv preprint directly added to the RT-qPCR reaction. Until more experiments are done to fully understand the intricacies of this effect, we opted for a compromise between increased capture efficiency and consistency and a weak suppressive effect on the reverse transcription efficiency. When applying these conditions to 204 clinical samples, we only observed a 2.5% inhibition of the RT-qPCR, indicating that our method is suitable for large-scale use in testing laboratories.
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