Author: Martin Mikl; Yitzhak Pilpel; Eran Segal
Title: High-throughput interrogation of programmed ribosomal frameshifting in human cells Document date: 2018_11_14
ID: 5zjnzsik_11
Snippet: Most single point mutations within the slippery sequence abrogated frameshifting and resulted in 253 green fluorescence at background levels, both for -1 and +1 frameshifting sites (Fig 2C, Fig S5E) , 254 demonstrating that we indeed measure frameshifting at the expected site. Replacing the last position 255 in the canonical slippery site X XXY YYZ had -on average -a less severe effect on PRF efficiency, 256 due to the fact that it is the wobble .....
Document: Most single point mutations within the slippery sequence abrogated frameshifting and resulted in 253 green fluorescence at background levels, both for -1 and +1 frameshifting sites (Fig 2C, Fig S5E) , 254 demonstrating that we indeed measure frameshifting at the expected site. Replacing the last position 255 in the canonical slippery site X XXY YYZ had -on average -a less severe effect on PRF efficiency, 256 due to the fact that it is the wobble position in the original frame and therefore has larger flexibility (Fig 257 2C ). In high efficiency PRF sites also this position showed strong and PRF site-specific preferences: 258
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