Author: Sun, Shi-Hui; Chen, Qi; Gu, Hong-Jing; Yang, Guan; Wang, Yan-Xiao; Huang, Xing-Yao; Liu, Su-Su; Zhang, Na-Na; Li, Xiao-Feng; Xiong, Rui; Guo, Yan; Deng, Yong-Qiang; Huang, Wei-Jin; Liu, Quan; Liu, Quan-Ming; Shen, Yue-Lei; Zhou, Yong; Yang, Xiao; Zhao, Tong-Yan; Fan, Chang-Fa; Zhou, Yu-Sen; Qin, Cheng-Feng; Wang, You-Chun
Title: A mouse model of SARS-CoV-2 infection and pathogenesis Cord-id: b6j89491 Document date: 2020_5_27
ID: b6j89491
Snippet: Summary Since December 2019, a novel coronavirus SARS-CoV-2 has emerged and rapidly spread throughout the world, resulting in a global public health emergency. The lack of vaccine and antivirals has brought an urgent need for animal model. Human Angiotensin converting enzyme II (ACE2) has been identified as a functional receptor for SARS-CoV-2. In this study, we generated a mouse model expressing human ACE2 (hACE2) using CRISPR/Cas9 knock-in technology. Compared with wild-type C57BL/6 mice, both
Document: Summary Since December 2019, a novel coronavirus SARS-CoV-2 has emerged and rapidly spread throughout the world, resulting in a global public health emergency. The lack of vaccine and antivirals has brought an urgent need for animal model. Human Angiotensin converting enzyme II (ACE2) has been identified as a functional receptor for SARS-CoV-2. In this study, we generated a mouse model expressing human ACE2 (hACE2) using CRISPR/Cas9 knock-in technology. Compared with wild-type C57BL/6 mice, both young and aged hACE2 mice sustained high viral loads in lung, trachea and brain upon intranasal infection. Although fatalities were not observed, interstitial pneumonia and elevated cytokines were seen in SARS-CoV-2 infected- aged hACE2 mice. Interestingly, intragastric inoculation of SARS-CoV-2 was evidenced to cause productive infection and lead to pulmonary pathological changes in hACE2 mice. Overall, this animal model described here provides a useful tool for studying SARS-CoV-2 transmission and pathogenesis, and evaluating COVID-19 vaccines and therapeutics.
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