Selected article for: "detection limit and real time pcr"
Author: Enora Dupas; Bruno Legendre; Valérie Olivier; Françoise Poliakoff; Charles Manceau; Amandine Cunty
Title: Comparison of real-time PCR and droplet digital PCR for the detection of Xylella fastidiosa in plants
  • Document date: 2019_3_20
    • ID: 0xc02kkb_14
    Snippet: Real-time PCR Harper et al. (2010) was used as a reference method in this study. For all the 312 assays, no mean Ct values exceeding 38 were recorded at a concentration equal to or higher 313 than the limit of detection, meaning that the limit of detection and positive results were 314 consistent. Moreover, for O. europaea the EPPO PM 7/24 protocol mentions a limit of detection 315 for samples artificially contaminated with Xf subsp. multiplex of.....
    Document: Real-time PCR Harper et al. (2010) was used as a reference method in this study. For all the 312 assays, no mean Ct values exceeding 38 were recorded at a concentration equal to or higher 313 than the limit of detection, meaning that the limit of detection and positive results were 314 consistent. Moreover, for O. europaea the EPPO PM 7/24 protocol mentions a limit of detection 315 for samples artificially contaminated with Xf subsp. multiplex of 100% at 1x10 5 b/mL. In this 316 study, the limit of detection of 5x10 4 b/mL was close to that presented in the PM 7/24 (EPPO, 317 2018b). The limit of detection of Xf in P. myrtifolia is known to be 1x10 3 , which is the same as 318 the value we found (Legendre B., personal communication). The limits of detection of Xf subsp. 319 multiplex for the other matrices could not be compared, as there are no available data. 320

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