Author: Ella N Hartenian; Britt A Glaunsinger
Title: Herpesvirus infection reduces Pol II occupancy of host promoters but spares viral promoters Document date: 2019_3_26
ID: i51almnu_16
Snippet: While widespread mRNA decay has been shown to facilitate immune evasion, gene expression 303 control, and latency establishment, the robust viral transcription we document here presents a 304 parsimonious explanation for a novel way in which the virus benefits from accelerated decay. buffer. Samples were eluted twice with 100 µL 5% Beta-mercaptoethanol (BME) in DEPC H20 358 for 5 min then RNA was precipitated with isopropanol and quantified by R.....
Document: While widespread mRNA decay has been shown to facilitate immune evasion, gene expression 303 control, and latency establishment, the robust viral transcription we document here presents a 304 parsimonious explanation for a novel way in which the virus benefits from accelerated decay. buffer. Samples were eluted twice with 100 µL 5% Beta-mercaptoethanol (BME) in DEPC H20 358 for 5 min then RNA was precipitated with isopropanol and quantified by RT-qPCR. 359
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