Author: Daniel J Butler; Christopher Mozsary; Cem Meydan; David C Danko; Jonathan Foox; Joel Rosiene; Alon Shaiber; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J Sedlazeck; Nikolay A Ivanov; Maria A Sierra; Diana Pohle; Michael Zeitz; Vijendra Ramlall; Undina Gisladottir; Craig D Westover; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Phyllis Ruggiero; Bradley W Langhorst; Nathan A Tanner; Justyn Gawrys; Dmitry Meleshko; Dong Xu; Jenny Xiang; Angelika Iftner; Daniela Bezdan; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari Melnick; Iman A Hajirasouliha; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F Westblade; Shawn Levy; Melissa Cushing; Nicholas P Tatonetti; Marcin Imielinski; Hanna Rennert; Christopher Mason
Title: Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Document date: 2020_4_20
ID: kyoa5gsf_5
Snippet: We evaluated the reproducibility, clinical sensitivity, and clinical specificity of the LAMP assay across a set of serial titrations. Across these experiments, LAMP fluorescence correlated closely with SARS-CoV-2 RNA viral copies (Figure 1d) , with an overlap of the median signal from negative controls at lower levels (25 total copies per reaction) of viral RNA (n=10) . This translated to a 100% reproducibility at 1,000 and 500 total copies of vi.....
Document: We evaluated the reproducibility, clinical sensitivity, and clinical specificity of the LAMP assay across a set of serial titrations. Across these experiments, LAMP fluorescence correlated closely with SARS-CoV-2 RNA viral copies (Figure 1d) , with an overlap of the median signal from negative controls at lower levels (25 total copies per reaction) of viral RNA (n=10) . This translated to a 100% reproducibility at 1,000 and 500 total copies of viral RNA, 90% reproducibility at 100 copies, and 80% reproducibility at 50 copies (Supp. Figure 3a) . This indicates an LoD threshold (95% reproducibility at two times the LoD) that is likely near 200 copies of RNA, with a maximum sensitivity of 10-50 copies of the viral RNA.
Search related documents:
Co phrase search for related documents- assay LAMP clinical specificity and clinical specificity: 1, 2, 3
- clinical sensitivity and closely correlate: 1
- clinical sensitivity and LAMP fluorescence: 1, 2, 3, 4
- clinical sensitivity and lod threshold: 1
- clinical sensitivity and low level: 1, 2
- clinical sensitivity and negative control: 1, 2, 3, 4
- clinical sensitivity and viral copy: 1, 2, 3, 4
- clinical specificity and LAMP fluorescence: 1
- clinical specificity and low level: 1
- clinical specificity and negative control: 1, 2, 3, 4
- clinical specificity and viral copy: 1, 2
- LAMP fluorescence and viral copy: 1
- lod threshold and viral copy: 1
- low level and median signal: 1, 2
- low level and negative control: 1, 2, 3
- median signal and negative control: 1
- negative control and viral copy: 1, 2
- reaction total copy and total copy: 1
- SARS RNA viral copy and viral copy: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date