Author: Chenyu Li; David N. Debruyne; Julia Spencer; Vidushi Kapoor; Lily Y. Liu; Bo Zhou; Lucie Lee; Rounak Feigelman; Grayson Burdon; Jeffrey Liu; Alejandra Oliva; Adam Borcherding; Hongdong Tan; Alexander E. Urban; Guoying Liu; Zhitong Liu
Title: High sensitivity detection of coronavirus SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method Document date: 2020_3_14
ID: 0hxan9rw_18
Snippet: In order to discover highly mutated viruses and unknown pathogens, we subsequently developed a userfriendly multiplex-PCR-based metagenomic method. In this method, random hexamer-adapters were used to amplify DNA or cDNA targets in a multiplex PCR reaction. The large amounts of non-specific amplification products were removed by using Paragon Genomics' proprietary background removing reagent, thus resolving a library suitable for sequencing. For .....
Document: In order to discover highly mutated viruses and unknown pathogens, we subsequently developed a userfriendly multiplex-PCR-based metagenomic method. In this method, random hexamer-adapters were used to amplify DNA or cDNA targets in a multiplex PCR reaction. The large amounts of non-specific amplification products were removed by using Paragon Genomics' proprietary background removing reagent, thus resolving a library suitable for sequencing. For RNA samples, our reverse transcription reagents were additionally used to convert RNA into cDNA, resulting in significantly reduced amount of human ribosomal RNA species.
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