Host protein CD63 enhances viral RNA replication by interacting with human astrovirus nonstructural protein nsP1a/4

wei zhao; Nian liu; Xiao Li Tao; Chun hong Zheng; Xiang yu Li; Man Yu; Yong gang Li

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/331835 doi: bioRxiv preprint treated with HRP-labeled goat anti-mouse IgG or goat anti-rabbit IgG as the The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/331835 doi: bioRxiv preprint with gentle shaking. The eluted proteins were detected by SDS-PAGE followed 399 by western blot analysis with anti-GST antibody and CD63 antibody. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/331835 doi: bioRxiv preprint pcDNA3.1-3flag-CD63 arelisted in Table 1 . Two pairs of shRNAs targeting 421 human CD63 and a negative control shRNA (Table 1) The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/331835 doi: bioRxiv preprint protein is involved in RNA replication. Virology 333(1), 124-31. Screen for interaction on synthetic dropout medium lacking tryptophan, 616 leucine,and histidine(SD-Trp-Leu-His). (C) Screen for interaction on synthetic 617 author/funder. All rights reserved. No reuse allowed without permission.