Selected article for: "High fidelity and supplementary table"
Author: Ben Longdon; Jonathan P Day; Joel M Alves; Sophia CL Smith; Thomas M Houslay; John E McGonigle; Lucia Tagliaferri; Francis M Jiggins
Title: Host shifts result in parallel genetic changes when viruses evolve in closely related species
  • Document date: 2017_11_28
    • ID: ithoxu6k_39
    Snippet: After passaging the virus, we sequenced evolved viral lineages from 19 host species, with a mean of 240 9 independent replicate lineages of the virus per species (range 6-10 replicates). cDNA was 241 synthesised using Invitrogen Superscript III reverse-transcriptase with random hexamer primers 242 (25°C 5mins, 50°C 50mins, 70°C 15mins). The genome of the evolved viruses, along with the initial 243 DCV ancestor (x5) were then amplified using Q5.....
    Document: After passaging the virus, we sequenced evolved viral lineages from 19 host species, with a mean of 240 9 independent replicate lineages of the virus per species (range 6-10 replicates). cDNA was 241 synthesised using Invitrogen Superscript III reverse-transcriptase with random hexamer primers 242 (25°C 5mins, 50°C 50mins, 70°C 15mins). The genome of the evolved viruses, along with the initial 243 DCV ancestor (x5) were then amplified using Q5 high fidelity polymerase (NEB) in nine overlapping 244 PCR reactions (see supplementary Table S2 for PCR primers and cycle conditions). Primers covered

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