Author: Mahesh K R Kalikiri; Mohammad Rubayet Hasan; Faheem Mirza; Thabisile Xaba; Patrick Tang; Stephan Lorenz
Title: High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads Document date: 2020_4_11
ID: bx2xspbe_6
Snippet: Across 47 total samples tested, the extraction method had 91% sensitivity, 100% specificity and 96% accuracy (Table 1 ) with 2 false negatives. All results produced by the new method were within <2 Cq of the validated in-house protocol (7) . Two samples that gave false negative results had Cq values ≥35, suggesting that the slightly lower sample volume that is fed into the extraction leads to slightly reduced sensitivity......
Document: Across 47 total samples tested, the extraction method had 91% sensitivity, 100% specificity and 96% accuracy (Table 1 ) with 2 false negatives. All results produced by the new method were within <2 Cq of the validated in-house protocol (7) . Two samples that gave false negative results had Cq values ≥35, suggesting that the slightly lower sample volume that is fed into the extraction leads to slightly reduced sensitivity.
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