Selected article for: "internal control and PCR inhibition"
Author: Mahesh K R Kalikiri; Mohammad Rubayet Hasan; Faheem Mirza; Thabisile Xaba; Patrick Tang; Stephan Lorenz
Title: High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads
  • Document date: 2020_4_11
    • ID: bx2xspbe_15
    Snippet: After establishing the protocol and implementing a version extracting 96 samples at a time on a Hamilton STAR, we tested 94 clinical samples side-by-side using the Biomérieux easyMAG system and the proposed method (Table 2) . Apart from one sample that tested weakly positive at Cq 38 for the validated method, all positive samples were correctly identified, with a Cq difference of <1 Cq. More importantly, only 3 out of 94 samples exhibited PCR in.....
    Document: After establishing the protocol and implementing a version extracting 96 samples at a time on a Hamilton STAR, we tested 94 clinical samples side-by-side using the Biomérieux easyMAG system and the proposed method (Table 2) . Apart from one sample that tested weakly positive at Cq 38 for the validated method, all positive samples were correctly identified, with a Cq difference of <1 Cq. More importantly, only 3 out of 94 samples exhibited PCR inhibition as determined by the successful detection of the internal control. Overall, the PCR inhibition rate was 3.2% for the proposed method, compared with 11.2% for the established commercial method. We also tested another subset of 204 pooled clinical samples (5 samples per pool), of which 69 (33.8%) tested positive, 130 (63.7%) negative, and 5 (2.5%) showed signs of PCR inhibition.

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