In vitro antiviral activity of stabilized chlorine dioxide containing oral care products

Shewale, Jaiprakash G; Gelhaus, H Carl; Ratcliff, James L; Hernandez-Kapila, Yvonne L

OBJECTIVE: To determine the antiviral activity in-vitro of oral care products containing stabilized chlorine dioxide towards infectious viruses that harbor in the oral cavity. METHODS: Validated in-vitro suspension virucidal assays were used. Test product was mixed with the test virus for 30-, 60- or 120- seconds, neutralized with sodium thiosulfate, serially diluted in dilution medium in a 96 well plate and incubated in a carbon dioxide incubator for 7 days. The 50% Tissue Culture Infectious Dose per mL was determined. RESULTS: Two rinses, one oral spray and one fluoride toothpaste showed log reduction of severe acute respiratory syndrome coronavirus-2 ranging from 1.81 to 2.98 and of influenza A from 2.58 to 4.13, respectively, within 30 seconds of contact time; similar results were obtained at 60 seconds. Further, the Ultra Sensitive rinse showed 0.19, 0.75, 1.58, 1.75, 2.66 and 3.24 log reduction of severe acute respiratory syndrome coronavirus, human coronavirus 229E, rhinovirus type 14, adenovirus type 5, and herpes simplex virus type 1 and type 2, respectively, within 30 seconds of contact time. CONCLUSION: Stabilized chlorine dioxide containing CloSYS® oral care products reduced the viral load of multiple viruses within 30 seconds. The results warrant further investigation for potential in-vivo applications.