Author: Mahesh K R Kalikiri; Mohammad Rubayet Hasan; Faheem Mirza; Thabisile Xaba; Patrick Tang; Stephan Lorenz
Title: High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads Document date: 2020_4_11
ID: bx2xspbe_29
Snippet: We used the QuantiFast Pathogen +IC (Qiagen) kit for the detection of the SARS-CoV-2 E gene and the internal control on a 7500 Fast RT-PCR instrument (Applied Biosystems). The master mix was created using 2.5 µl 5x QuantiFast Pathogen master mix, 0.1 µl 100x QuantiFast RT mix, 0.25 µl 50x ROX dye solution and 0.9 µl RNAsefree water and 3.5 µl primer-probe mix (to final concentration of 0......
Document: We used the QuantiFast Pathogen +IC (Qiagen) kit for the detection of the SARS-CoV-2 E gene and the internal control on a 7500 Fast RT-PCR instrument (Applied Biosystems). The master mix was created using 2.5 µl 5x QuantiFast Pathogen master mix, 0.1 µl 100x QuantiFast RT mix, 0.25 µl 50x ROX dye solution and 0.9 µl RNAsefree water and 3.5 µl primer-probe mix (to final concentration of 0.
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