Author: Martin Mikl; Yitzhak Pilpel; Eran Segal
Title: High-throughput interrogation of programmed ribosomal frameshifting in human cells Document date: 2018_11_14
ID: 5zjnzsik_3
Snippet: and 5922 (44%) sequences cloned into the -1 PRF and +1 PRF reporter, respectively. 100 Mean and 95% CI of -1 (C) and +1 (D) PRF signal (% GFP fluorescence) for barcode control groups corresponding to the indicated wild-type PRF sequences (blue), carrying single nucleotide deletions (green) or having a stop codon inserted upstream of the frameshift sequence (red); n between 1 and 32; the shaded area denotes the range of background fluorescence; as.....
Document: and 5922 (44%) sequences cloned into the -1 PRF and +1 PRF reporter, respectively. 100 Mean and 95% CI of -1 (C) and +1 (D) PRF signal (% GFP fluorescence) for barcode control groups corresponding to the indicated wild-type PRF sequences (blue), carrying single nucleotide deletions (green) or having a stop codon inserted upstream of the frameshift sequence (red); n between 1 and 32; the shaded area denotes the range of background fluorescence; asterisks denote significant differences between the wild-type and the other groups (single nucleotide deletion and stop-control) combined (Mann-Whitney-U test). E. Comparison of -1 and +1 PRF reporter readout (% GFP fluorescence) for previously reported PRF sites (cf. Fig 1CD, Fig S3AC) .
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