Selected article for: "agarose gel electrophoresis and Extraction kit"
Author: Martin Mikl; Yitzhak Pilpel; Eran Segal
Title: High-throughput interrogation of programmed ribosomal frameshifting in human cells
  • Document date: 2018_11_14
    • ID: 5zjnzsik_38
    Snippet: of 72•C for 5min. Specific primers corresponding to the constant region upstream and downstream of 825 the splice sites were used. The PCR products were separated from potential unspecific fragments by 826 electrophoresis on a 1.5% agarose gel stained with EtBr, cut from the gel, and cleaned in 2 steps: gel 827 extraction kit (Qiagen) and SPRI beads (Agencourt AMPure XP). The sample was assessed for size 828 and purity at the Tapestation, using.....
    Document: of 72•C for 5min. Specific primers corresponding to the constant region upstream and downstream of 825 the splice sites were used. The PCR products were separated from potential unspecific fragments by 826 electrophoresis on a 1.5% agarose gel stained with EtBr, cut from the gel, and cleaned in 2 steps: gel 827 extraction kit (Qiagen) and SPRI beads (Agencourt AMPure XP). The sample was assessed for size 828 and purity at the Tapestation, using high sensitivity D1K screenTape (Agilent Technologies, Santa 829 Clara, California). We used 20 ng library DNA for library preparation for NGS; specific Illumina 830 adaptors were added, and DNA was amplified using 14 amplification cycles. The sample was 831 reanalyzed using Tapestation. 832

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