Author: lianhua dong; Junbo Zhou; Chunyan Niu; Quanyi Wang; Yang Pan; Xia Wang; Yongzhuo zhang; Jiayi yang; Manqing Liu; Yang zhao; Tao peng; Jie xie; yunhua gao; Di wang; yun zhao; Xinhua Dai; xiang fang
Title: Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR Document date: 2020_3_18
ID: aa7slcnc_12
Snippet: The linear range was investigated by varying the mean copy number per droplet, denoted as λ (16). The precision or relative error of RT-dPCR is related to λ gene assay. To determine the lower limit of all three assays, serial dilutions of each RNA transcript were prepared (Supplemental Table 1 ). The measured targets matched the anticipated values in each tested interval. A good linearity (0.93
Document: The linear range was investigated by varying the mean copy number per droplet, denoted as λ (16). The precision or relative error of RT-dPCR is related to λ gene assay. To determine the lower limit of all three assays, serial dilutions of each RNA transcript were prepared (Supplemental Table 1 ). The measured targets matched the anticipated values in each tested interval. A good linearity (0.93
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