Author: Clayton M. Carey; Sarah E. Apple; Zoe A. Hilbert; Michael S. Kay; Nels C. Elde
Title: Conflicts with diarrheal pathogens trigger rapid evolution of an intestinal signaling axis Document date: 2020_3_30
ID: ju826pao_31
Snippet: Organoids used for analysis were identified in ImageJ and assigned ROI identifiers so that the same organoids could be easily assessed across timepoints. 30 organoids from each strain were selected randomly to include a variety of starting sizes and were checked for GC-C expression by comparison with GFP images. GC-C expressing organoids that ruptured during the experiment were excluded from analysis. Swelling was assessed by measuring the area o.....
Document: Organoids used for analysis were identified in ImageJ and assigned ROI identifiers so that the same organoids could be easily assessed across timepoints. 30 organoids from each strain were selected randomly to include a variety of starting sizes and were checked for GC-C expression by comparison with GFP images. GC-C expressing organoids that ruptured during the experiment were excluded from analysis. Swelling was assessed by measuring the area of each organoid in ImageJ at t=0 and t=120 min. The percent change in organoid area from 0 to 120 min was calculated for each organoid and plotted in Prism. Significance in organoid swelling data was assessed by an unpaired t-test with Welch's correction using Prism 8. Figure S1 : Sequence conservation in primate guanylin and uroguanylin. Amino acid sequence alignments of the secreted forms of primate guanylin and uroguanylin are shown. Sequences were retrieved from genbank and aligned using clustal omega. The sequence of marmoset uroguanylin (C. jacchus), was unavailable.
Search related documents:
Co phrase search for related documents- amino acid and clustal omega: 1, 2, 3, 4, 5, 6
- amino acid and sequence alignment: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77
- amino acid and sequence conservation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- analysis exclude and sequence alignment: 1
- clustal omega and sequence alignment: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- clustal omega and sequence conservation: 1
Co phrase search for related documents, hyperlinks ordered by date