Author: lianhua dong; Junbo Zhou; Chunyan Niu; Quanyi Wang; Yang Pan; Xia Wang; Yongzhuo zhang; Jiayi yang; Manqing Liu; Yang zhao; Tao peng; Jie xie; yunhua gao; Di wang; yun zhao; Xinhua Dai; xiang fang
Title: Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR Document date: 2020_3_18
ID: aa7slcnc_5
Snippet: The primer and probe sequences for detecting N and ORF1ab gene target of the SARS-CoV-2 published by Chinese CDC were used for this study (12) . for 30 sec. The cycled plate was then transferred to the QX200 Droplet Reader (Bio-Rad) and analyzed using the QuantaSoft droplet reader software (V1.7.4, Bio-Rad). Reactions containing more than 10,000 droplets were treated as effective and involved in data analysis......
Document: The primer and probe sequences for detecting N and ORF1ab gene target of the SARS-CoV-2 published by Chinese CDC were used for this study (12) . for 30 sec. The cycled plate was then transferred to the QX200 Droplet Reader (Bio-Rad) and analyzed using the QuantaSoft droplet reader software (V1.7.4, Bio-Rad). Reactions containing more than 10,000 droplets were treated as effective and involved in data analysis.
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