A nematode-specific gene underlies bleomycin-response variation in Caenorhabditis elegans

Shannon C. Brady; Stefan Zdraljevic; Karol W. Bisaga; Robyn E. Tanny; Daniel E. Cook; Daehan Lee; Ye Wang; Erik C. Andersen

We identified all single nucleotide variants (SNVs), small insertion/deletions (indels), and 646 structural variants (SVs) present in these 83 strains for which the CB4856 strain contains the 647 alternate allele compared to the N2 reference strain. We found 105 variants within the QTL 648 confidence interval (79 SNVs, 26 indels, 0 SVs) for which the CB4856 strain contains the 649 alternate allele (Figure S15, File S20) . We then identified SNVs and indels with a minor-allele 650 frequency less than 5% within the 83 strains, because these low-frequency variants are likely to 651 have insufficient power to map by GWA. Seventy-two of the 105 variants in the region were 652 identified as rare variants that might underlie the bleomycin-response difference between the N2 653 and CB4856 strains (Figure S15, File S20) . Twenty-eight of these rare variants were not unique 654 . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/565218 doi: bioRxiv preprint