Author: lianhua dong; Junbo Zhou; Chunyan Niu; Quanyi Wang; Yang Pan; Xia Wang; Yongzhuo zhang; Jiayi yang; Manqing Liu; Yang zhao; Tao peng; Jie xie; yunhua gao; Di wang; yun zhao; Xinhua Dai; xiang fang
Title: Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR Document date: 2020_3_18
ID: aa7slcnc_2
Snippet: Digital PCR (dPCR) is a technology which partitions nucleic acid molecules across a large number of smaller reactions and acquires amplification data of each partition at end point based on the intensity of fluorescence (7-9). Quantification is performed by applying Poisson statistics to the proportion of the negative partitions to account for positive partitions that initially contained more than one target molecule. dPCR can offer greater preci.....
Document: Digital PCR (dPCR) is a technology which partitions nucleic acid molecules across a large number of smaller reactions and acquires amplification data of each partition at end point based on the intensity of fluorescence (7-9). Quantification is performed by applying Poisson statistics to the proportion of the negative partitions to account for positive partitions that initially contained more than one target molecule. dPCR can offer greater precision than qPCR and is far simpler to use for copy number quantification due the binary nature in which the partitions are counted as positive or negative. Additionally, dPCR is more tolerant of PCR inhibition compared with qPCR due to partitioning and because it is an end-point PCR measurement and consequently less dependent on high PCR efficiency (10, 11) .
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