Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics.

Lee, Youngseop; Kang, Byoung-Hoon; Kang, Minhee; Chung, Doo Ryeon; Yi, Gwan-Su; Lee, Luke P; Jeong, Ki-Hun

Emerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases in the point-of-care test. Here we report nanoplasmonic on-chip PCR for rapid precision molecular diagnostics. The nanoplasmonic pillar arrays (NPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light-emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between the nanoislands. As a result, they effectively induce photothermal heating for ultrafast PCR thermal cycling. The temperature profile of NPA exhibit 30 cycles between 98 ℃ and 60 ℃ for total 3 min 30 sec during the cyclic excitation of white LED light. The experimental results also demonstrate the rapid DNA amplification of both 0.1 ng μl-1 of λ-DNA in 20 thermal cycles and 0.1 ng μl-1 of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles using a conventional PCR volume of 15 μl. This nanoplasmonic PCR technique provides a new opportunity for rapid precision molecular diagnostics.