Author: Ting Gao; Mingdong Hu; Xiaopeng Zhang; Hongzhen Li; Lin Zhu; Hainan Liu; Qincai Dong; Zhang Zhang; Zhongyi Wang; Yong Hu; Yangbo Fu; Yanwen Jin; Kaitong Li; Songtao Zhao; Yongjiu Xiao; Shuping Luo; Lufeng Li; Lingfang Zhao; Junli Liu; Huailong Zhao; Yue Liu; Weihong Yang; Jing Peng; Xiaoyu Chen; Ping Li; Yaoning Liu; Yonghong Xie; Jibo Song; Lu Zhang; Qingjun Ma; Xiuwu Bian; Wei Chen; Xuan Liu; Qing Mao; Cheng Cao
Title: Highly pathogenic coronavirus N protein aggravates lung injury by MASP-2-mediated complement over-activation Document date: 2020_3_30
ID: dxs8ggyh_7
Snippet: The CCP1-CCP2-SP domains of MASP-2 are responsible for self-activation and 35 substrate binding activity, which in turn mediates complement lectin pathway activation (23). The MASP-2: N protein association demonstrated above suggests that SARS-CoV N protein may regulate MASP-2 dimerization, activation and cleavage. To demonstrate MASP-2 dimerization, MASP-2-Flag-conjugated beads were incubated with lysates of 293T cells expressing MASP-2-Myc in t.....
Document: The CCP1-CCP2-SP domains of MASP-2 are responsible for self-activation and 35 substrate binding activity, which in turn mediates complement lectin pathway activation (23). The MASP-2: N protein association demonstrated above suggests that SARS-CoV N protein may regulate MASP-2 dimerization, activation and cleavage. To demonstrate MASP-2 dimerization, MASP-2-Flag-conjugated beads were incubated with lysates of 293T cells expressing MASP-2-Myc in the presence/absence of N protein and MBL. Binding of MASP-2-(residues 445-686) resulting from MASP-2 auto-activation were produced in the presence of SARS-CoV N protein (Fig. 2B) .
Search related documents:
Co phrase search for related documents- bind activity and substrate bind activity: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date