Author: Cerrato, Andrea; Capriotti, Anna Laura; Montone, Carmela Maria; Aita, Sara Elsa; Cannazza, Giuseppe; Citti, Cinzia; Piovesana, Susy; Aldo, LaganÃ
Title: Analytical Methodologies for Lipidomics in Hemp Plant. Cord-id: bs5tmmn3 Document date: 2021_1_1
ID: bs5tmmn3
Snippet: The chemical composition of Cannabis sativa L. has been extensively studied for tens of years, but little is known about its lipidome. This chapter describes an analytical workflow for polar lipid determination in hemp. After extraction, lipids are enriched and isolated by graphitized carbon black sorbent, and the isolated lipid is analyzed by liquid chromatography (LC) coupled with high resolution mass spectrometry, leading to identification of many lipid species. We have developed a semi-autom
Document: The chemical composition of Cannabis sativa L. has been extensively studied for tens of years, but little is known about its lipidome. This chapter describes an analytical workflow for polar lipid determination in hemp. After extraction, lipids are enriched and isolated by graphitized carbon black sorbent, and the isolated lipid is analyzed by liquid chromatography (LC) coupled with high resolution mass spectrometry, leading to identification of many lipid species. We have developed a semi-automated platform using commercially available Lipostar software for lipid identification. Our approach affords the identification of 189 polar lipids in hemp extract, including sulfolipids and phospholipids. The number of the identified lipid species is by far the highest ever reported for Cannabis sativa.
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