Author: Nicola F Fletcher; Luke W Meredith; Emma L Tidswell; Steven R Bryden; Daniel Gonçalves-Carneiro; Yasmin Chaudhry; Claire Shannon-Lowe; Michael A Folan; Daniella A Lefteri; Marieke Pingen; Dalan Bailey; Clive S McKimmie; Alan W Baird
Title: A novel antiviral formulation inhibits a range of enveloped viruses. Document date: 2020_3_30
ID: nly9vojr_32
Snippet: For non-enveloped viral infectivity assays, a similar approach was used to enveloped virus assays. ViroSAL was mixed with murine norovirus (MNV-CW1) in a 1:1 ratio, incubated at room temperature for 2 minutes and the pH was restored to 7. Virus infection of permissive RAW 264.7 (mouse macrophage) and BV2 (murine microglial) cells were conducted immediately after neutralization in triplicate, incubated at 37°C and quantified 96h post infection us.....
Document: For non-enveloped viral infectivity assays, a similar approach was used to enveloped virus assays. ViroSAL was mixed with murine norovirus (MNV-CW1) in a 1:1 ratio, incubated at room temperature for 2 minutes and the pH was restored to 7. Virus infection of permissive RAW 264.7 (mouse macrophage) and BV2 (murine microglial) cells were conducted immediately after neutralization in triplicate, incubated at 37°C and quantified 96h post infection using TCID50 via microscopic visualization for the appearance of cytopathic effect.
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